Abstract Roadkill is one of the most direct impacts on fauna, leading to numerous direct and indirect impacts. Road-killed animals could serve as a resource for research and teaching purposes, but first, they need to have their tissues preserved by some technique. Plastination is one of the most modern techniques for the conservation of biological tissues, its principle is the replacement of body fluids by a curable polymer, resulting in a non-toxic, odorless, and manipulable material. So the main aim of this work was to compose a collection of plastinated roadkill victims from the Biological Reserve Sooretama region, adapting the standard protocol to the different taxa worked here (Avialae, Reptilia, Amphibia, and Mammalia). For this, 42 animals were used, previously frozen and stored in the Sooretama Biological Reserve and Vale Natural Reserve until they were sent for processing. Upon arriving at the Plastination laboratory at the Federal University of Espírito Santo, the animals underwent the 4 stages of plastination: fixation, dehydration, forced impregnation, and chemical cure; some adaptations were made to improve the final specimens. The results proved that the plastination of these animals is extremely viable in generating satisfactory results. Given the results obtained, it becomes clear how plastination can become an alternative to implement in the composition of biological collections using roadkill animals. The animals prepared in this work comprise an unprecedented collection available for different applications, such as exhibitions, practical classes, and research.

ABSTRACT Insects are fundamental to biodiversity conservation in almost all ecosystems, and their population decline, and extinction directly result from environmental impacts. These facts are aggravated by the lack of knowledge of insect biodiversity, the so-called biodiversity deficits, especially the Linnean and Wallacean shortfalls. In freshwater ecosystems, biodiversity loss is higher among aquatic insects, and caddisflies comprise one of the most vulnerable orders. In this way, research focusing on describing new caddisfly species and understanding their distribution ranges will increase knowledge of caddisfly biodiversity. In the past 14 years, the team from the Laboratório de Entomologia Aquática “Prof. Dr. Claudio Gilberto Froehlich” (LEAq, Universidade Federal da Bahia, Brazil) and collaborators have been addressing these issues, resulting in 55 described species of caddisflies. Taxa in other insect orders have also received attention and an additional 16 species have been described (eight mayflies, four stoneflies, four neuropteran spongillaflies, and antlions). Here, eight caddisfly species are described and illustrated (Atopsyche froehlichi sp. nov., Austrotinodes zeferina sp. nov., Cernotina kariri sp. nov., Neoathripsodes froehlichi sp. nov., Notalina claudiofroehlichi sp. nov., Oecetis marcus sp. nov., Phylloicus froehlichi sp. nov., and Polycentropus claudioi sp. nov.), five of them in honor of LEAq’s patron. In addition, new distributional data are presented for 10 known species. A checklist of the caddisfly fauna of Bahia state is also presented, with 138 species, around 75% and 30% of them recorded and described by the LEAq team, respectively. Currently, for caddisflies, Bahia is the fifth most species-rich state in Brazil, and the first in the Brazilian Northeast region.

ABSTRACT. The objective of this research was to identify the influence of the evaporative adiabatic cooling system (EACS) on the thermal comfort and productive responses of dairy cattle, through multivariate analysis by principal components (PC), in the summer and winter seasons of the semiarid region of Pernambuco, Brazil. The data came from an experiment that included 16 multiparous lactating cows (7/8 Holstein-Gyr), randomly distributed in 4 sets, with 4 experimental phases and 4 treatments (0, 10, 20, and 30 min.). The multivariate analysis was carried out through PC for the thermal comfort indices, physiological variables, and milk production and composition. The highest milk production in the summer season was obtained for animals exposed to the cooling system for 30 min. In the winter season in the morning period, the use of the EACS for 10 min. was sufficient for improvements in milk production. The times of exposure to EACS caused changes in the composition of milk, for both seasons. Principal component analysis made it possible to verify a positive correlation of evaporative cooling with thermal comfort, physiological responses, and production and composition of milk of lactating cows.

ABSTRACT Clinical similarities among viral diseases become even more relevant considering the current scenario, especially in Brazil, where there is a high incidence of these diseases and overlapping seasonality. We report the case of a patient with acute clinical manifestations composed of predominant respiratory symptoms and alveolar hemorrhage in which three etiologies (dengue, influenza and COVID-19) were investigated concomitantly. Only the diagnosis of dengue was confirmed. Then, the patient’s immunological profile in response to stimulation of mononuclear cells with dengue virus antigen was analyzed in an attempt to identify specific characteristics that could be associated with the clinical manifestation.

ABSTRACT Background: Domestic pigeons carry pathogens in their droppings, posing a potential public health problem. Methods: The phenotypic and genotypic antimicrobial resistances of Staphylococcus aureus and Enterococcus faecium in the feces of urban pigeons near hospitals with intensive care units were measured. Results: Twenty-nine samples showed Enterococcus growth, whereas one was positive for S. aureus. The S. aureus isolate was sensitive to the antibiotics tested via antibiogram, however resistance genes were identified. E. faecium isolates showed phenotypic resistance to gentamicin, erythromycin, and ciprofloxacin. Conclusions: Antimicrobial profiles harmful to health were demonstrated in bacterial pathogens isolated from the external environment of hospitals.

RESUMO A presença de anormalidades no alinhamento dos joelhos (geno varo e geno valgo) é extremamente comum no futebol. Seu aparecimento pode ocorrer quando os jogadores estão nas categorias de base e se perpetuar durante a carreira profissional. As consequências de anos de prática esportiva em alto nível em jogadores com geno varo/geno valgo podem ser deletérias aos joelhos. A partir da observação de jogos de futebol do presente e do passado, os autores do artigo notaram que grandes nomes brasileiros deste esporte foram ou são acometidos pelo desalinhamento dos joelhos: Garrincha - o mais famoso -, Leandro, Dunga, Romário, Rivaldo e Neymar. O presente estudo tem como objetivo discutir a relação entre o esporte de alto rendimento e o desenvolvimento dessas deformidades ortopédicas, servindo como ferramenta de atualização da subespecialidade traumato-ortopédica do joelho. Nível III de Evidência, Estudo Retrospectivo Comparativo.

ABSTRACT The presence of abnormalities in knee alignment (genu varum and genu valgum) is extremely common in soccer. It can occur in the junior league / youth academy and perpetuate itself throughout the professional career. The consequences of years of high-level sports practice in players with genu varum / genu valgum can be harmful to the knees. By observing football matches of the present and the past, the authors of the article noted that great Brazilian soccer players were, or are, affected by the malalignment of the knees: Garrincha (the most famous), Leandro, Dunga, Romário, Rivaldo and Neymar. This study aims to discuss the relationship between high-performance sport and the development of these orthopedic deformities, serving as a tool for updating the trauma-orthopedic knee subspecialty. Level of Evidence III, Retrospective comparative study.

Resumen El objetivo fue caracterizar térmicamente las instalaciones durante las fases de crecimiento y finalización de los cerdos y evaluar la influencia de los sistemas de control climático en la expresión de los comportamientos de los cerdos. El experimento se realizó en la Universidad Federal Rural de Pernambuco, Serra Talhada, PE, Brasil, durante la primavera de 2019. Se utilizaron 27 cerdos de 63 días. Los animales fueron sometidos a corrales sin sistema de climatización, con ventilación forzada y con sistema de enfriamiento adiabático evaporativo. Se registraron la temperatura de bulbo seco (° C), la humedad relativa del aire (%), la temperatura del globo negro (° C) y las respuestas de comportamiento de los cerdos, sometidas a análisis multivariante, utilizando la técnica de componentes principales. Los corrales con sistema de enfriamiento adiabático evaporativo mostraron una reducción significativa de la temperatura del aire y un aumento de la humedad relativa del aire, durante las dos fases. Los cerdos sometidos a enfriamiento evaporativo mostraron mayor actividad ingestiva y comportamiento letárgico, propio de las fases estudiadas.

Abstract The objective was to thermally characterize the facilities during the growth and finishing phases of pigs and to evaluate the influence of climate control systems on the expression of the behaviors of pigs. The experiment was conducted at the Federal Rural University of Pernambuco, Serra Talhada, PE, Brazil, during the spring of 2019. Twenty-seven pigs aged 63 days were used. The animals were submitted to pens without climate control system, with forced ventilation, and with an evaporative adiabatic cooling system. Dry bulb temperature (°C), relative air humidity (%), black globe temperature (°C), and swine behavioral responses were recorded, submitted to multivariate analysis, using the principal component technique. Pens with the evaporative adiabatic cooling system showed a significant reduction in air temperature and an increase in relative air humidity, during the two phases. Pigs submitted to evaporative cooling showed greater ingestive activity and lethargic behavior, typical of the studied phases.

ABSTRACT The objective of this study was to estimate the breed, heterosis, and recombination effects on different components of the lactation curve of Girolando cattle. The dataset used consisted of 12,121 purebred cows of Holstein (H) and Gyr (G) breeds, and six H×G crossbred cows (Girolando). The model used presents random effects of herd and cow, regression coefficient associated with linear effect of proportion of H breed, regression coefficient associated with the linear effect of heterosis between H and G breeds, regression coefficient associated with the linear effect of recombination between H and G breeds, and random effect of residual. Dijkstra's (DJ), Nelder's (ND), Wilmink's (WL), and Wood's (WD) models were tested to fit production records of these different genetic groups. These models were then tested according to evaluation criteria of quality of fit (AIC, BIC, and RMSE), and the two best models (WD and WL) were chosen for estimation of 305-day milk yield (MY305), peak yield, time to peak, and persistency of milk yield. The breed effect was significant for all traits and components of the lactation curve. The heterosis effect was significant for all traits, and was more significant for MY305 (945.62±79.17 kg). Peak yield was the component of lactation curve that presented the most significant heterosis effect, partially explaining the heterosis effect (12 to 21%) found for MY305. The recombination effect was positive only for lactation period and time to peak of lactation in Girolando cows.

Resumo Objetivou-se avaliar a resposta da atividade biológica medida pela técnica Biospeckle Laser aplicada a mangas (Mangifera indica L.) submetidas a danos mecânicos, em diferentes estágios de maturação. Os testes foram realizados duas vezes por semana, durante 21 dias, sendo utilizados 40 frutos de manga da variedade Tommy. O procedimento foi dividido em duas etapas: na primeira etapa, simulou-se o impacto nos frutos; na segunda etapa, foi aplicada a luz laser sobre a área que sofreu impacto, seguido do registro das imagens de todos os frutos. Esses registros foram submetidos a um processamento gráfico, com a finalidade de se obterem mapas de atividade biológica dos frutos. A técnica Biospeckle laser associada à análise de imagem permitiu identificar níveis de atividades biológicas distintas nas mangas, certificando o seu potencial de aplicação como ferramenta de teste não destrutivo, no monitoramento da atividade biológica em amostras de frutos.

Abstract The objective of this study was to evaluate the response of biological activity measured by the Biospeckle Laser technique applied to mangoes (Mangifera indica L.) subjected to mechanical damage at different stages of maturation. The tests were carried out twice a week for twenty-one days, and 40 mangoes of the variety Tommy Atkins were used. The procedure was divided into two stages: the first simulated the impact on fruits, and in the second stage, the laser light was applied to the impacted area. After laser application, the images of all fruits were submitted to graphic processing, in order to obtain maps of biological activity of the fruits. The Biospeckle laser technique associated with image analysis allowed the identification of distinct biological activity levels in the mangoes, certifying its potential application as a non-destructive test tool, in the monitoring of biological activity in fruit samples.

ABSTRACT. This study aimed to analyze the principal components of the meteorological variables, physiological and behavioral response of cows subjected to different cooling times and their influence on milk quality, in the dry and rainfall periods, and to establish multiple regression models for milk quality. The data used in the study came from an experiment conducted in the Agreste Region of Pernambuco. The pre-milking cooling time was 10, 20, 30 min. and the control (without cooling). Sixteen multiparous lactating Gir cows were selected. Data were analyzed by principal component analysis and a multiple regression analysis was applied to determine milk quality. There was a strong relationship between somatic cell count (SCC) and activity of the animal in the shade for dry, and lying for rainfall, with increased SCC in cow milk. It was possible to establish two multiple regression models to determine milk quality in dry and rainfall periods. According to the principal component analysis, the cooling time to meet the thermal requirement of the animals was 20 min., regardless of the season and milking shift.

Resumen La región semiárida brasileña se caracteriza por la escasez de agua, lo que provoca la pérdida de hojas de la vegetación nativa para reducir la transpiración. Con la reducción del área foliar de Caatinga el suelo está más expuesto, convertirme en un gran aliado para la degradación ambiental. El objetivo fue vigilar y analizar la dinámica espacio-temporal de la vegetación de Caatinga mediante la teledetección orbital en región semiárida de Pernambuco, Brasil, en la estación de lluvias en la estación seca. Estudio se desarrolló mediante imágenes del satélite Landsat-8, entre los años 2013-2018, y mediante el algoritmo SEBAL se determinaron mapas temáticos de los parámetros biofísicos: albedo y temperatura superficial, índice de vegetación de diferencia normalizada (NDVI), índice de vegetación ajustado al suelo (SAVI) e índice de área foliar (LAI). Los resultados muestran que en la estación seca hay una aptitud más notable para que se produzca la degradación ambiental.

Abstract The Brazilian semiarid region is marked by water scarcity, which causes the loss of leaves from native vegetation to reduce transpiration. With the reduction of the Caatinga leaf area, the soil becomes more exposed, which makes it a great ally for environmental degradation. This study aimed to monitor and analyze the spatial-temporal dynamics of the Caatinga vegetation by orbital remote sensing in the semiarid region of Pernambuco, Brazil, in the rainy and dry season. The study was developed from Landsat-8 satellite images between the years 2013-2018. From the SEBAL algorithm, thematic maps of the biophysical parameters were determined: albedo and surface temperature, normalized difference vegetation index (NDVI), soil-adjusted vegetation index (SAVI), and leaf area index (LAI). The results show that in the dry season, there is a greater aptitude for environmental degradation to occur.

BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples.

ABSTRACT. The aim of this study was to employ the principal component technique to physiological data and environmental thermohygrometric variables correlated with detection of clinical and subclinical mastitis in dairy cattle. A total of 24 lactating Girolando cows with different clinical conditions were selected (healthy, and with clinical or subclinical mastitis). The following physiological variables were recorded: udder surface temperature, ST (°C); eyeball temperature, ET (°C); rectum temperature, RT (°C); respiratory frequency, RF (mov. min-1). Thermohygrometric variables included air temperature, AirT (°C), and relative humidity, RU (%). ST was determined by means of thermal images, with four images per animal, on these quarters: front left side (FL), front right side (FR), rear right side (RR) and rear left side (RL), totaling 96 images. Exploratory data analysis was run through multivariate statistical technique with the employment of principal components, comprehending nine variables: ST on the FL, FR, RL and RR quarters; ET, RT; RF, AirT and RU. The representative quarters of the animals with clinical and subclinical mastitis showed udder temperatures 8.55 and 2.46° C higher than those of healthy animals, respectively. The ETs of the animals with subclinical and clinical mastitis were, respectively, 7.9 and 8.0% higher than those of healthy animals. Rectum temperatures were 2.9% (subclinical mastitis) and 5.5% (clinical mastitis) higher compared to those of healthy animals. Respiratory frequencies were 40.3% (subclinical mastitis) and 61.6% (clinical mastitis) higher compared to those of healthy animals. The first component explained 91% of the total variance for the variables analyzed. The principal component technique allowed verifying the variables correlated with the animals' clinical condition and the degree of dependence between the study variables.

RESUMO Objetivos: Analisar os resultados dos exames de pré-participação dos atletas de um clube de futebol profissional, visando confrontar os achados cardiográficos com a literatura e incentivar o desenvolvimento de novas estratégias para a prevenção da morte súbita. Método: Utilizamos uma amostra com 110 atletas, jogadores de futebol do sexo masculino. Etapas do estudo: 1) coleta de dados dos exames de pré-participação (anamnese cardiológica, eletrocardiograma, teste ergométrico e ecocardiograma), através de formulário, em um período de três anos (2015 a 2017); 2) tabulação dos dados utilizando os softwares Word e Excel Office 2010; 3) confrontação com a literatura. Resultados: Dos atletas estudados, 55,5% apresentaram bradicardia sinusal e 14,5% alteração na repolarização ventricular, 33,3% evidenciaram refluxo tricúspide mínimo e 45,7% apresentaram refluxo pulmonar fisiológico. O ecocardiograma apresentou alguns dados interessantes quando comparados à população adulta não atleta. No teste ergométrico, atingiram o estágio máximo 53,6% dos atletas e 46,4% interromperam o teste por cansaço físico. Em relação às arritmias, em 21,8% observaram-se extrassístoles ventriculares isoladas e raras e em 8,2% extrassístoles supraventriculares isoladas e raras. Conclusões: Os achados reforçam os dados da literatura de cardiologia do exercício e esporte, pois, representam, em sua maioria, adaptações fisiológicas do coração do atleta. Cabe ao médico do esporte o acompanhamento clínico dos atletas, visando prevenção da morte súbita. Nível de Evidência II; Estudo retrospectivo.

ABSTRACT Objectives: To analyze the results of pre-participation tests applied to soccer players from a professional club, aiming to compare the cardiographic findings with the literature and encourage the development of new strategies for the prevention of sudden death. Methods: We used a sample group of 110 male soccer players. Stages of the study: 1) collection of data from the pre-participation tests (cardiac history, electrocardiogram, exercise test and echocardiogram) using a form covering three years (2015 to 2017); 2) tabulation of data using Word and Excel Office 2010 software; 3) comparison with the literature. Results: Of the athletes studied, 55.5% had sinus bradycardia and 14.5% had ventricular repolarization abnormalities, 33.3% showed evidence of minimal tricuspid regurgitation, and 45.7% had physiological pulmonary regurgitation. The echocardiogram presented some interesting data when compared to the adult non-athlete population. In the ergometric test, 53.6% of the athletes reached the maximum stage and 46.4% discontinued the test due to physical fatigue. Regarding arrhythmias, in 21.8% of the patients we observed rare isolated ventricular extrasystoles and in 8.2% rare isolated supraventricular extrasystoles. Conclusion: The findings corroborate data from the literature on exercise and sports cardiology, since they mainly represent physiological adaptations of the athlete's heart. The sports physician is responsible for monitoring athletes to prevent sudden death. Level of Evidence II; Retrospective study.

RESUMEN Objetivos: Analizar los resultados de los exámenes de pre participación de los atletas de un club de fútbol profesional, con el fin de confrontar los hallazgos cardiográficos con la literatura e incentivar el desarrollo de nuevas estrategias para la prevención de la muerte súbita. Método: Utilizamos una muestra de 110 atletas, jugadores de fútbol del sexo masculino. Etapas del estudio: 1) colecta de datos de estos exámenes de pre participación (anamnesis cardíaca, electrocardiograma, test ergométrico y ecocardiograma), a través de formulario, en un período de tres años (2015 a 2017); 2) tabulación de los datos utilizando los softwares Word y Excel Office 2010; 3) confrontación con la literatura. Resultados: De los atletas estudiados, 55,5% presentaron bradicardia sinusal y 14,5% alteración en la repolarización ventricular, 33,3% evidenció reflujo tricúspide mínimo y 45,7% presentaron reflujo pulmonar fisiológico. El ecocardiograma presentó algunos datos interesantes cuando comparados a la población adulta no atleta. En el test ergométrico, alcanzaron la etapa máxima 53,6% de los atletas y 46,4% interrumpió la prueba por cansancio físico. Con cuanto a las arritmias, en 21,8% se observaron extrasístoles ventriculares aisladas y raras, y en 8,2% extrasístoles supraventriculares aisladas y raras. Conclusión: Los hallazgos refuerzan los datos de la literatura de cardiología del ejercicio y deporte, pues representan en su mayoría, adaptaciones fisiológicas del corazón del atleta. Cabe al médico del deporte el acompañamiento clínico de los atletas buscando la prevención de la muerte súbita. Nivel de Evidencia II; Estudio retrospectivo.

RESUMO Objetivou-se com essa pesquisa avaliar o padrão de variabilidade espacial da temperatura de superfície do úbere de vacas leiteiras saudáveis e com mastite. Foram selecionados 24 animais, oito saudáveis, oito com mastite subclínica e oito com mastite clínica. As imagens foram obtidas a partir de uma câmera termográfica Flir i60, resolução de 0,01 °C, 1,0 m de distância focal e emissividade ajustada para 0,98. O horário de realização das imagens foi entre às 05 e 07h00, que totalizaram 96 imagens, três por animal, nos enquadramentos anterolateral direito, anterolateral esquerdo, posterior e inferior. As análises foram realizadas pela geoestatística, com ajuste do semivariograma para validação do modelo teórico e construção dos mapas de krigagem. A temperatura média de superfície dos quartos mamários com classificação positiva para mastite subclínica apresentou valores entre 33,2 ± 0,67ºC e 34,64± 1,07ºC; para os quartos negativos entre 29,3 ± 1,78ºC e 32,24 ± 0,62ºC. Os animais saudáveis apresentaram temperatura de superfície de úbere inferior àqueles com mastite subclínica (29,3ºC ± 1,78 e 31,58ºC ± 0,62). A temperatura da superfície do úbere dos animais com mastite clínica foi mais elevada, entre 34,0 e 37,5°C, comparativamente aos demais quadros clínicos. A escala adotada para análise do padrão das imagens identificou com sucesso a dependência espacial da temperatura de superfície do úbere, o que contribuiu para padronização dos procedimentos de diagnóstico para animais saudáveis, com mastite subclínica e clínica, por meio da geoestatística.

ABSTRACT The objective of this research was to assess the spatial variability pattern concerning udder surface temperature in dairy cows that were healthy and in those with mastitis. A total of 24 animals were selected - eight healthy, eight with subclinical mastitis, and eight with clinical mastitis. Images were taken with a Flir i60 thermographic camera - resolution of 0.01°C, focal length of 1.0 m, and emissivity adjusted to 0.98 - between 05:00 and 07:00, totaling 96 images, three per animal, of the front and rear, right and left mammary quarters. Analyses were run through geostatistics, with semivariogram adjustment to validate the theoretical model and build kriging maps. The average surface temperature of the mammary quarters with positive classification for subclinical mastitis stood between 33.2 ± 0.67ºC and 34.64± 1.07ºC; for negative quarters, values ranged from 29.3 ± 1.78ºC to 32.24 ± 0.62ºC. The udder surface temperatures of healthy animals were lower than those of animals with subclinical mastitis (29.3ºC ± 1.78 and 31.58ºC ± 0.62). The udder surface temperature of animals with clinical mastitis was higher, between 34.0 and 37.5°C, compared to the other clinical statuses. The scale adopted for image pattern analysis successfully identified the spatial dependence of udder surface temperature, which helped standardize diagnostic procedures for healthy animals, and for those with subclinical and clinical mastitis, by means of geostatistics.